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Flow cytometric histogram analyses of cell surface marker expressions of eBMSCs at Passage 4: All eBMSC donors expressed CD29, CD44, CD90, and <t>CD105</t> while being negative for CD73 and CD79a. Donors 1, 2, 3, and 6 were negative for CD45, whereas donors 4, and 5 were slightly positive for CD45. Red histograms represent unstained controls and black histogram represent stained samples. eBMSCs: Equine bone marrow-derived bone marrow-derived mesenchymal stem cells
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Antibodies used for surface characterization.

Journal: Current Issues in Molecular Biology

Article Title: Cisplatin-Mediated IL-6 and IDO1 Suppression in Mesenchymal Stromal Cells: Implications for Tumor Microenvironment Modulation In Vitro

doi: 10.3390/cimb47040231

Figure Lengend Snippet: Antibodies used for surface characterization.

Article Snippet: FITC mouse anti-human CD105 (555690) , Becton Dickinson GmbH, Heidelberg, Germany.

Techniques:

MSC-typical constellation of six surface antigens was evaluated by flow cytometry. Here, the constellation of three donors is shown. Donors 4, 5 and 6 were included (n = 3), negativity for CD31 ( A , G , M ), CD34 ( B , H , N ), CD45 ( C , I , O ), positivity for CD73 ( D , J , P ), CD90 ( E , K , Q ), CD105 ( F , L , R ).

Journal: Current Issues in Molecular Biology

Article Title: Cisplatin-Mediated IL-6 and IDO1 Suppression in Mesenchymal Stromal Cells: Implications for Tumor Microenvironment Modulation In Vitro

doi: 10.3390/cimb47040231

Figure Lengend Snippet: MSC-typical constellation of six surface antigens was evaluated by flow cytometry. Here, the constellation of three donors is shown. Donors 4, 5 and 6 were included (n = 3), negativity for CD31 ( A , G , M ), CD34 ( B , H , N ), CD45 ( C , I , O ), positivity for CD73 ( D , J , P ), CD90 ( E , K , Q ), CD105 ( F , L , R ).

Article Snippet: FITC mouse anti-human CD105 (555690) , Becton Dickinson GmbH, Heidelberg, Germany.

Techniques: Flow Cytometry

Flow cytometric histogram analyses of cell surface marker expressions of eBMSCs at Passage 4: All eBMSC donors expressed CD29, CD44, CD90, and CD105 while being negative for CD73 and CD79a. Donors 1, 2, 3, and 6 were negative for CD45, whereas donors 4, and 5 were slightly positive for CD45. Red histograms represent unstained controls and black histogram represent stained samples. eBMSCs: Equine bone marrow-derived bone marrow-derived mesenchymal stem cells

Journal: BMC Veterinary Research

Article Title: Comparison of the chondrogenic potential of eBMSCs and eUCMSCs in response to selected peptides and compounds

doi: 10.1186/s12917-024-04448-3

Figure Lengend Snippet: Flow cytometric histogram analyses of cell surface marker expressions of eBMSCs at Passage 4: All eBMSC donors expressed CD29, CD44, CD90, and CD105 while being negative for CD73 and CD79a. Donors 1, 2, 3, and 6 were negative for CD45, whereas donors 4, and 5 were slightly positive for CD45. Red histograms represent unstained controls and black histogram represent stained samples. eBMSCs: Equine bone marrow-derived bone marrow-derived mesenchymal stem cells

Article Snippet: CD105 , Mouse anti-human CD105 antibody-clone SN6-Reacts with horse , Biorad , MCA1557SBV440 , [ ] .

Techniques: Marker, Staining, Derivative Assay

Flow cytometric histogram analyses of cell surface marker expressions of (eUCMSCs) at Passage 4: All eUCMSC donors expressed CD29, CD44, CD90, and CD105, while being negative for CD45, and CD73. Donors 1, and 3 were slightly positive for CD79A. Red histograms represent unstained controls and black histogram represent stained samples. eUCMSCs: Equine umbilical cord-derived mesenchymal stromal cells

Journal: BMC Veterinary Research

Article Title: Comparison of the chondrogenic potential of eBMSCs and eUCMSCs in response to selected peptides and compounds

doi: 10.1186/s12917-024-04448-3

Figure Lengend Snippet: Flow cytometric histogram analyses of cell surface marker expressions of (eUCMSCs) at Passage 4: All eUCMSC donors expressed CD29, CD44, CD90, and CD105, while being negative for CD45, and CD73. Donors 1, and 3 were slightly positive for CD79A. Red histograms represent unstained controls and black histogram represent stained samples. eUCMSCs: Equine umbilical cord-derived mesenchymal stromal cells

Article Snippet: CD105 , Mouse anti-human CD105 antibody-clone SN6-Reacts with horse , Biorad , MCA1557SBV440 , [ ] .

Techniques: Marker, Staining, Derivative Assay

Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Journal: BMC Veterinary Research

Article Title: Comparison of the chondrogenic potential of eBMSCs and eUCMSCs in response to selected peptides and compounds

doi: 10.1186/s12917-024-04448-3

Figure Lengend Snippet: Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Article Snippet: CD105 , Mouse anti-human CD105 antibody-clone SN6-Reacts with horse , Biorad , MCA1557SBV440 , [ ] .

Techniques:

Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Journal: BMC Veterinary Research

Article Title: Comparison of the chondrogenic potential of eBMSCs and eUCMSCs in response to selected peptides and compounds

doi: 10.1186/s12917-024-04448-3

Figure Lengend Snippet: Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Article Snippet: CD105 , Mouse anti-human CD105 antibody-clone SN6-Reacts with horse , Biorad , MCA1557SBV440 , [ ] .

Techniques:

Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Journal: BMC Veterinary Research

Article Title: Comparison of the chondrogenic potential of eBMSCs and eUCMSCs in response to selected peptides and compounds

doi: 10.1186/s12917-024-04448-3

Figure Lengend Snippet: Antibodies used for flow cytometric analysis of cell surface markers of eBMSCs and eUCMSCs

Article Snippet: CD105 , Mouse anti-human CD105 antibody-clone SN6-Reacts with horse , Biorad , MCA1557SBV440 , [ ] .

Techniques:

Mean percentage expression of CD29, CD44, CD73, CD90,  CD105,  CD34, and CD45 in MLC-N and MLC-OA across three passages, as measured by flow cytometry.

Journal: International Journal of Molecular Sciences

Article Title: Disease-Associated Signatures Persist in Extracellular Vesicles from Reprogrammed Cells of Osteoarthritis Patients

doi: 10.3390/ijms26030870

Figure Lengend Snippet: Mean percentage expression of CD29, CD44, CD73, CD90, CD105, CD34, and CD45 in MLC-N and MLC-OA across three passages, as measured by flow cytometry.

Article Snippet: FITC Mouse Anti-Human CD105 , 1:50 , Human Endoglin (ENG) , SN6 , AbD Serotec.

Techniques: Expressing, Cytometry

Antibodies conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), or PE/Cy5 used for flow cytometry.

Journal: International Journal of Molecular Sciences

Article Title: Disease-Associated Signatures Persist in Extracellular Vesicles from Reprogrammed Cells of Osteoarthritis Patients

doi: 10.3390/ijms26030870

Figure Lengend Snippet: Antibodies conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), or PE/Cy5 used for flow cytometry.

Article Snippet: FITC Mouse Anti-Human CD105 , 1:50 , Human Endoglin (ENG) , SN6 , AbD Serotec.

Techniques: Cytometry, Control